Driving from Chennai straight to the court, 60-year-old Sasikala appeared before the special court judge Ashwathnarayana, as the Supreme Court refused to give her a breather rejecting her request for more time to surrender.
Travelling in the same car that Jayalalithaa used, Sasikala arrived at the central jail housed at Parappana Agrahara, close to Hosur on Karnataka-Tamil Nadu border, towards the evening amid tight security.
Sasikala's relatives V N Sudhakaran and Elavarasi, who were also convicted by the trial court, were also imprisoned after they surrendered and fulfilled court formalities.
She had earlier spent 21 days in Parappana Agrahara jail after conviction by the trial court in September 2014.
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Sasikala and Elavarasi would share a small cell in women's block in the jail, Rao said.
He said Sasikala would get normal food, not home food, but it would be according to the doctor's advice.
Sudhakaran would also be sharing the cell with other inmates, he said.
In commotion after Sasikala's arrival, four cars in her cavalcade were damaged in vandalism, police said but added it was not known who did it.
Thousands had gathered then in an emotionally charged atmosphere with AIADMK leaders and MLAs in large numbers but this time it was a subdued atmosphere, with only the media contingent, onlookers and a group of supporters being present.
With the Supreme Court being firm on not giving any more time, Sasikala left Jayalalithaa's Poes Garden residence in Chennai just before noon and headed to Bengaluru after visiting Jayalalithaa's memorial on Marina Beach where she paid floral tributes to the late leader.
Sasikala had yesterday given pep talk to her party MLAs
and supporters to stay bold and remain united.
"Only I can be jailed, not the care or concern I have for this party. Wherever I will be, my thoughts will be here," she had told both her MLAs at Koovathur resort as well as to her supporters later at Poes Garden late last night.
She would be thinking about the party round-the-clock and that "no force can wean away" the party from her, Sasikala, whose chief ministerial ambitions were put paid to by the Supreme Court verdict, had said.
At the Jayalalithaa memorial, Sasikala made her way through the crowd of supporters that included a sobbing former minister Gokula Indira and Deputy General Secretary and her nephew T T V Dinakaran, and put up a brave face.
Some women there also performed 'aarti' for Sasikala.
During the brief hearing today, the Supreme Court said the word 'immediately' has been used in its judgement with regard to the surrender of the convict.
"I hope you (Sasikala's counsel KTS Tulsi) understand the meaning of the word 'immediately'," Justice P C Ghose who headed the two-member bench said.
The trial court had found disproportionate assets worth Rs 53.60 crore, which Jayalalithaa and the three others could not account for. The CBI had alleged that the unaccounted wealth was in the tune of Rs 66.65 crore.
The top court had directed Sasikala and Sudhakaran, Jayalalithaa's disowned foster son, and Elavarasi, widow of Sasikala's elder brother, to "forthwith surrender" before the trial court which will "take immediate steps" to ensure that they all "serve out the remainder of sentence awarded to them and take further steps in compliance of this judgement, in accordance with law."
The proceedings against Jayalalithaa were abated as she breathed her last on December 5 last year.
Jayalalithaa was disqualified following her conviction in the case by the trial court on September 27, 2014, when Special Court Judge Michael D Cunha held her and three others guilty of corruption and awarded four years jail term.
The apex court verdict means Sasikala cannot hold public office or contest elections for 10 years, the period of her jail term plus six years after that.