Sasikala left from Poes Garden and headed for the resort located at Koovathur, about 80 km from here, and will hold "key discussions" with the MLAs, party-backed Jaya TV said.
She had held discussions with her MLAs at the resort on Saturday and Sunday and stressed the need to stay united to form the government.
On February 5, Sasikala was elected as the party's Legislature Leader, a step towards her elevation as Tamil Nadu Chief Minister.
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Later, addressing the AIADMK MLAs, Sasikala indicated that she could stay back for the night at the resort when she said she had brought a set of dresses and that "we could return together tomorrow".
Repeatedly invoking the legacy of party veterans, late MG Ramachandran and late J Jayalalithaa, she said AIADMK workers would work by their ideals of public service.
Sasikala said a grand memorial will be built at the burial site of Jayalalithaa, which will "put everybody in awe".
Sasikala charged those in the Panneerselvam camp with intimidating her party MLAs, especially the women, and asked if this was right on the part of the chief minister as he also held the police portfolio and was entrusted with upholding law-and-order in the state.
"But, we should be calm. This is our rule and we will rule for the rest of the term," she said, adding that the party workers will do every bit to uphold the legacy of MGR and Jayalalithaa.
She recalled Jayalalithaa uniting the AIADMK after MGR's demise and steering it through difficult times.
Earlier, Sasikala visited the houses of local villagers and interacted with them. She also named a girl child after Jayalalithaa.