The donation follows a USD 500 million Saudi gift to the UN effort to help Iraqi refugees fleeing a jihadist advance.
"We have been stung by the evil of terrorism," Saudi Ambassador to the United States Adel bin Ahmed Al-Jubeir told reporters.
"We believe that those countries that have not been stung by terrorism seem to be lax in dealing with terrorism in a serious way," he told reporters afer presenting a cheque to UN chief Ban Ki-moon.
The United States, Germany and Britain have also donated to help run the UN centre.