"It is known that women in Saudi are banned from driving and laws will be applied against violators and those who demonstrate in support" of this cause, interior ministry spokesman General Mansur al-Turki told AFP.
Activists have called on social networks for Saudi women, individually, to go behind the wheel on Saturday, in a campaign in the world's only country that bans women from driving.
Yesterday, the interior ministry issued a statement saying it would crack down against anyone who attempts to "disturb public peace" by congregating or marching "under the pretext of an alleged day of female driving."
It added that the interior ministry "will fully and firmly enforce the laws against violators".
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Turki insisted that "all gatherings are prohibited" in Saudi Arabia.
Women who defied the law in the past ran into trouble with the authorities and were harassed by compatriots.
In 1990, authorities stopped 47 women who got behind the wheel in a demonstration against the driving ban
In 2011, activist Manal al-Sharif, one of the organisers of this Saturday's campaign, was arrested and held nine days for posting online a video of herself behind the wheel.
Activists have repeatedly insisted throughout their campaign that no demonstrations will be held.
"October 26 is a day on which women in Saudi Arabia will say they are serious about driving and that this matter must be resolved," the Dubai-based Sharif has told AFP about the weekend protest.
She said women have begun responding to the call and over the past two weeks have posted videos online showing women already driving in Saudi Arabia.