The research used observations from NASA's Wide-field Infrared Survey Explorer (WISE) orbiting observatory.
"The idea behind our research is that, if an entire galaxy had been colonised by an advanced spacefaring civilisation, the energy produced by that civilisation's technologies would be detectable in mid-infrared wavelengths - exactly the radiation that the WISE satellite was designed to detect for other astronomical purposes," said Jason T Wright at Penn State University.
Roger Griffith, the lead author of the paper at Penn State, scoured almost the entire catalogue of the WISE satellite's detections - nearly 100 million entries - for objects consistent with galaxies emitting too much mid-infrared radiation.
He then individually examined and categorised around 100,000 of the most promising galaxy images.
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"Our follow-up studies of those galaxies may reveal if the origin of their radiation results from natural astronomical processes, or if it could indicate the presence of a highly advanced civilisation," he said.
"Our results mean that, out of the 100,000 galaxies that WISE could see in sufficient detail, none of them is widely populated by an alien civilisation using most of the starlight in its galaxy for its own purposes," Wright said.
"That's interesting because these galaxies are billions of years old, which should have been plenty of time for them to have been filled with alien civilisations, if they exist. Either they don't exist, or they don't yet use enough energy for us to recognise them," Wright said.
"This cluster is probably a group of very young stars forming inside a previously undiscovered molecular cloud, and the 48 Librae nebula apparently is due to a huge cloud of dust around the star, but both deserve much more careful study," said Matthew Povich, an assistant professor of astronomy at Cal Poly Pomona, and a co-investigator on the project.