The coach is also blaming the some of the team's struggles, which include a disappointing performance that nearly led to its elimination by Chile in the second round, on the pressure of needing to succeed in front of the home fans.
Brazil has only six players with experience of past World Cups. Some of its top stars, including Neymar, are playing on football's biggest stage for the first time.
"Of course it's a concern, we have a young team," Scolari said after Brazil's win over Chile in a penalty shootout.
The veteran coach used the example of young midfielder Willian, who is one of the team's best penalty takers in training but sent his shot wide in Saturday's shootout against Chile in Belo Horizonte.
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"We will be gaining more experience in each match we play," Scolari said. "Hopefully, in the next matches we will be making fewer mistakes because of this lack of experience. We can't lose anymore."
Scolari said Brazil's disappointing second-half performance against Chile can be blamed on the pressure felt by the players, who started feeling more and more anxious as the match neared its end and elimination became a possibility.
Several Brazilian players were crying after the match was over, including Neymar and captain Thiago Silva. Goalkeeper Julio Cesar, who made two penalty saves, had watery eyes even before the shootout started.