Deputy Superintendent of Police (DSP) Majeed Abbasi was shot dead by assailants while he was travelling in a police mobile van in Karachi's Shah Latif Town area.
"He was posted as SP of Site town and going for duty when the assailants attacked him and he died later in a hospital," IG police Ghulam Qadir Thebo told the media.
Thebo said four assailants had gunned down Abbasi.
Last month, unidentified target killers gunned down two DSPs in different incidents in Orangi town and Shah Faisal colony.
Prime Minister Nawaz Sharif strongly condemned Abbasi's murder and sought a report.
The Premier also expressed his concern over the number of police officials targeted in Karachi in the last few months.