The 30-share barometer fell by 100.77 points or 0.39 per cent to 25,703.01, with indices -- IT, auto, consumer durable, metal and bank -- leading the fall.
The index had gained 759.35 points in the last four sessions.
Also, the NSE Nifty was trading lower by 35.10 points or 0.44 per cent at 7,809.35.
Brokers said that apart from profit-booking in recent gainers, weak trend at other Asian bourses, tracking overnight losses at the US markets, mainly influenced the sentiment.
The Dow Jones Industrial Average ended 1.43 per cent lower in yesterday's trade.