The 30-share index, which had gained 166.30 points in the previous session, fell by 171.85 points or 0.61 per cent to 27,724.12.
Selling in realty, banking, power, capital goods, metal, PSU, oil&gas and auto stocks weighed on the performance.
On similar lines, the NSE Nifty was trading lower by 45.50 points, or 0.54 per cent, at 8,352.50.
Brokers said besides a weak trend in global markets after another round of Greek debt reform talks ended without agreement just days before it must repay a debt to avoid default and possible euro zone exit, selling by investors despite beginning of the July F&O series, dragged down the indices.
The US Dow Jones Industrial Average ended 0.42 per cent lower in yesterday's trade.