The 30-share barometer, which had lost 280.62 points in the previous two sessions, moved down by another 61.40 points, or 0.29 per cent, to 25,929.83 with capital goods, realty, metal and healthcare stocks leading the fall.
Also, the National Stock Exchange index Nifty shed 18.60 points, or 0.24 per cent, to 7,730.10.
Brokers said offloading of positions by foreign funds and retail investors in view of tomorrow's monthly expiry in the derivatives segment influenced the trading sentiment.
Stock of Larsen and Toubro plunged 7.98 per cent to Rs 1,513.45 after the company's quarterly earnings did not meet market expectations.
Among other Asian markets, Japan's Nikkei rose 0.27 per cent, while Hong Kong's Hang Seng was trading 0.75 per cent higher in early trade.
The US Dow Jones Industrial Average ended 0.42 per cent down in yesterday's trade.