The 30-share barometer, which had gained 6.58 points in yesterday's choppy trade, moved down by 77.78 points, or 0.32 per cent, to 24,478.31 with IT, auto, PSUs, metal and banking stocks leading the fall.
Also, the National Stock Exchange index Nifty shed 13.25 points, or 0.18 per cent, to 7,316.40.
Brokers said offloading of positions by foreign funds and retail investors on the last session of May's expiry in the derivatives segment and mixed cues from other Asian markets influenced the trading sentiments.
Among other Asian markets, Japan's Nikkei fell 0.58 per cent, while Hong Kong's Hang Seng was trading 0.22 per cent higher in early trade.
The US Dow Jones Industrial Average ended 0.25 per cent down in yesterday's trade.