The 30-share barometer, which lost 235.77 points in the previous two sessions, fell further by 110.29 points, or 0.39 per cent, to 27,721.25.
Stocks of metal, FMCG, auto, power, capital goods and banking sectors led the fall.
The wide-based National Stock Exchange index Nifty fell 40.60 points, or 0.47 per cent, to 8,425.95.
Brokers said a weak trend in Asian markets with Shanghai continuing a sharp sell-off on concerns the world's number two economy is slowing dampened trading sentiment here.
The US Dow Jones Industrial Average ended 0.19 per cent down in yesterday's trade.