Late sell-off was seen in pharma, IT, banking, power and capital goods, which pulled down the key indices.
Sentiment decidedly remained downbeat, hit by muted earnings by some blue-chips and sustained capital outflows, brokers said.
India's services sector activity rose to an 8-month high in October, powered by a significant jump in new business orders even as growth in manufacturing output eased, a Nikkei survey said, which contained any further damage.
The index had risen 31.44 points in yesterday's choppy session on value-buying in recently-battered stocks.
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The 50-issue NSE Nifty found itself at the receiving end of profit-booking and fell to 8,040.20 at the close, a loss of 20.50 points, or 0.25 per cent.
"Indices opened on a strong note in line with their strong global peers. However, last half hour of selling pressure dragged indices sharply lower as investors seemed worried about the crucial Bihar election result," said Gaurav Jain, Director, Hem Securities.
Sun Pharma, RIL, Lupin, NTPC, Cipla and Infosys too ended in the red.
Among the 30-Sensex stocks, 19 ended lower.
On a sectoral basis, the BSE healthcare index plunged the most by losing 0.79 per cent, followed by IT, banking, power and capital goods.
Broader markets offered a mixed picture. The mid-cap index, continued its upward journey and ended 0.19 per cent higher while small-cap shed 0.33 per cent.
Foreign investors sold shares worth Rs 449.84 crore yesterday, provisional data showed.