Besides, ending monthly expiry in the derivatives segment also weighed on the trading sentiments.
The 30-share barometer fell by 58.85 points, or 0.31 per cent, to 18,645.68 with realty, auto and FMCG sector stocks leading the fall. The index had gained 23.11 points in the previous session on Tuesday.
Similarly, the wide-based National Stock Exchange index Nifty fell by 18.55 points, or 0.33 per cent, to 5,623.05.
Brokers said fresh selling by funds and retail investors on worries over domestic political stability and a weak trend in the Asian region in line with overnight fall on the US markets dampened the trading sentiment.
Besides, monthly expiry in the derivatives segment too generated selling, they said.
In the Asian region, Hong Kong's Hang Seng index down 0.33 per cent, while Japan's Nikkei fell 1.56 per cent in early trade today. The US Dow Jones Industrial Average ended 0.23 per cent lower in yesterday's trade.