The 30-share barometer declined 43.81 points, or 0.17 per cent, to 25,241.56, with IT, banking, technology, FMCG, and infrastructure stocks taking a big knock.
The index had gained 608 points in the last two sessions on increasing prospects of a lower policy rate and sustained foreign fund inflows.
Also, the NSE Nifty cracked below the 7,700-mark by falling 31.30 points, or 0.41 per cent, at 7,672.95.
Brokers said that apart from profit-booking in recent gainers, mixed trend in other Asian bourses influenced sentiment.
The Dow Jones Industrial Average ended 0.12 per cent higher in yesterday's trade.