The 30-share barometer dropped by 160.09 points, or 0.63 per cent, to 25,102.12, with all sectoral indices led by FMCG, metal, IT and auto stocks leading the fall with losses up to 1.08 per cent.
The index had gained 160.48 points in the previous session.
Also, the broader NSE Nifty dipped below the crucial 7,700-mark by falling 51.10 points, or 0.66 per cent to 7,684.40.
Stocks of ITC group plunged 1.98 per cent to Rs 311.25 after the company said its cigarette factories have been shut since May 4 and will remain so till the time it is able to comply with "interim requirement" of 85 per cent pictorial warnings.
Also Read
Shares of country's largest two-wheeler maker Hero MotoCorp fell 1.15 per cent to Rs 2,860.75 despite the company's 70.85 per cent rise in standalone net profit at Rs 814.16 crore for the fourth quarter ended March 31.
Brokers said apart from profit-booking , a weak trend at other Asian bourses as investors await the release of key US jobs data later in the day, dampened sentiment here.
In the Asian region, Hong Kong's Hang Seng was down 1.27 per cent and Japan's Nikkei fell 0.72 per cent. Shanghai Composite fell 1.44 per cent in early trade.
The Dow Jones Industrial Average, however, ended marginally up by 0.05 per cent in yesterday's trade.