Realty, power and banking counters saw much of the losses as investors felt that the recent rally is "overdone".
Globally, it was a mixed picture. While Europe was off to a lower start, the rest of Asia closed higher mirroring an overnight rally in the US on an unexpected services sector pick-up and signs of a revival in oil prices.
"As markets take a pause ahead of earnings season, Brexit fears and shaky European markets continue to send shock waves. Yesterday's US ADP report lent a positive bias early in the day, but market will wait for Friday data to recalibrate its expectations for a December US rate hike," said Anand James, Chief Market Strategist, Geojit BNP Paribas Financial Services.
The 50-share NSE Nifty remained listless too and ended down 34.40 points, or 0.39 per cent, at 8,709.55.
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In the Sensex pack, NTPC plunged the most by 2.42 per cent, followed by Cipla 2.30 per cent. Others that ended up in the red include M&M, Power Grid, ICICI Bank, SBI, Infosys and Dr Reddy's, falling by up to 1.90 per cent.
Realty sector took the maximum hit as it fell 1.48 per cent, followed by power (1.18 per cent), healthcare (0.99 per cent) and IT (0.87 per cent).
Sentiment took a knock after selling picked up towards the fag end of the session, particularly in realty, power, banking and healthcare stocks, as investors rushed to lock in profits.
Broader markets were no better, with the mid-cap and small-cap indices falling 0.56 per cent and 0.48 per cent, respectively.
Foreign portfolio investors (FPIs) purchased shares worth a net Rs 243.00 crore yesterday, showed provisional data.
"Despite starting the day on a positive note, benchmark
indices failed to hold above the dotted line and succumbed to profit booking. Both the Sensex and the Nifty finally closed the day with losses of over 0.25 per cent," said Karthikraj Lakshmanan, Senior Fund Manager - Equities, BNP Paribas Mutual Fund.
"Institutional activity continued in a similar vein and the pace of selling by foreign institutional investors slowed considerably in yesterday's trade.