The "one-day mass causal leave" is likely to cripple settlement activities at the banks.
The unions are opposing the government move to take away the public debt management activities from the Central bank and curtail its powers on the monetary policy.
The protest, the first at the Central bank in six years, has been called by the United Forum of Reserve Bank Officers and Employees, which is the umbrella organisation of four recognised unions of officers and workmen at the Central bank.
These unions together have around 17,000 members associated with it.
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"The cease-work programme is intended, inter alia, to strongly oppose the government's current moves to cripple the RBI in the name of the draft financial code and legislative reforms," the United Forum had said in a release earlier.
The Finance ministry is giving the final touches to shift government's debt management functions from RBI to the proposed Public Debt Management Agency (PDMA), which will also henceforth function as depository of government securities (G-Secs), thus taking away from the RBI some vital operations having relevance to money market as well, the Forum added.
The union is opposing the proposed creation of a monetary policy committee that would include members of the government, seeing it as a curb on the RBI's decision-making powers.
The top management led by governor Raghuram Rajan has been in support of the new monetary policy framework as well as creation of the PDMA.