The Sharif family's spokesperson said that the prime minister's kin do not own and run any businesses in India.
"Imran Khan should not misguide the nation by propaganda," said the spokesman.
He said that Imran should be "ashamed" of spreading baseless allegations and lies.
"Imran Khan has time and again claimed that the Sharif family has businesses in India, although the allegations have always been denied by the latter as baseless," he was quoted as saying by Geo News.
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Yousaf Abbas Sharif had also said that not a single Indian is working in Sharif family sugar mill.
Earlier this year, Prime Minister's son Hussain Nawaz denied any links to business partnerships in India.
Hussain had said that his family neither owns any commercial business in India, nor are they involved in a business partnership with any Indian industrialist anywhere in the world.
In 2015, Imran had claimed that Sharif has earned USD 60 million from his business with India including setting up of two new sugar mills.
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