Desai was speaking to reporters after filing his nomination for the March 23 Rajya Sabha election from Maharashtra.
"Our stand on him is loud and clear. We have voiced it openly several times," Desai said, when asked if Sena members would vote for Rane if its ally, BJP, were to field him.
"If there is to be a change in the stand, the decision will be taken by Shiv Sena chief Uddhav Thackeray," Desai said.
Earlier, the Sena had opposed the proposal to induct Rane, who quit the Congress last year and declared support for the NDA, into the state cabinet.
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Desai, if he gets elected, will enter the Upper House of Parliament for a second time. He is the only candidate fielded by Sena for the Rajya Sabha election.
While he needs votes of 42 MLAs, the Sena has 63 MLAs in the state.
The BJP has decided to field three candidates from Maharashtra while it has 122 MLAs. Which means it would need four extra votes.
Rane quit the Congress last year and floated a new party while declaring support for the NDA.
The election for six RS seats from Maharashtra will be held on March 23.
Presently, the Congress and the NCP each hold two of these six seats while the BJP and Shiv Sena share the other two.
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