Chief Minister K Chandrasekhar Rao gave orders to this effect, a release from his office said here.
The shops and other establishments used to remain closed on Sundays and other holidays earlier, but they were allowed to remain open and transact business throughout the year after the formation of Telangana (in June 2014), it said.
The policy was implemented for a year on experimental basis and it came to an end yesterday, it said.
As per Rao's directives, the Labour Department issued orders to protect the rights of workers.
Accordingly, the shops should function only from 9 AM to 11 PM and a worker should work for a maximum of eight hours a day and 48 hours in a week, it said.
Workers are entitled for one holiday per week. Other measures for the welfare of workers, including women, have also been announced.