The ban would be in force from 6 pm tomorrow to 6 am local time next Monday in the Little India precinct, where weekend alcohol ban is imposed during weekends following the December 8 riot involving some 400 migrant workers from South Asia.
Thaipusam marks the anniversary when Lord Murugan received a Vel (spear) from his mother Goddess Parvathi so he could vanquish an evil demon.
It also commemorates Lord Shiva's dance of the 'ananda tandava' that revealed His form of Nataraja at the hallowed Shiva temple of Chidambaram in Tamil Nadu.
The Thaipusam procession would begin tomorrow 11 pm local time from Sri Srinivasa Perumal Temple at Serangoon Road in Little India and end at Sri Thendayuthapani Temple at Tank Road, just off the central business district.
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Individual group of Hindu worshipers would continue the procession in turn through Friday 11.59 pm local time.
K Shekaran, chairman of Sri Srinivasa Perumal Temple, said, "We have informed all devotees through guidelines that consumption of alcohol and smoking is not permitted within the processions route.
Alcohol ban was introduced to restore law and order after the riot in Little India, which gets crowded over the weekends and during holidays with migrant workers from South Asia spending their day off amidst a cluster of Indian-origin businesses, eateries and pubs.
The spontaneous riot on December 8 was sparked by an accident in which an Indian national was killed.
It was Singapore's worst street violent in 40 years.
Twenty-five Indian nationals are facing riot charges in court.