The research led by University of Western Sydney studied 63,048 Australian males aged 45-64.
The research was part of the 45 and Up Study, the largest long-term study of ageing in Australia, involving more than 267,000 people.
Study participants were asked to report on a range of health-related variables including the presence or absence of chronic diseases, and their daily sitting time.
Sitting time was divided into four categories: less than four hours, four to six hours, six to eight hours, and more than eight hours.
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George said that compared with those who spent four hours or less sitting down each day, participants reporting higher amounts of sitting were much more likely to report serious health conditions.
"The rates of chronic diseases reported by the participants exponentially increased in proportion with the amount of time the participants spent sitting down," said George.
Independent of factors such as age, Body Mass Index and level of physical activity, the amount of time men spent sitting each day was significantly associated with chronic disease and diabetes - indicating that, despite how active people may be outside of work hours, the amount of time spent sitting during the day may still have a significant impact.
She said the study is highly relevant to office workers and anyone whose daily job requires them to sit down for long periods of time, such as truck drivers.
"People should consider ways that they can integrate movement into their daily routines. Perhaps arranging 'active' meetings rather than a teleconference, or walk around during your lunch break rather than sitting at your desk," she added.
The study was published in the International Journal of Behavioral Nutrition and Physical Activity.