But the turnout on a clear, cold day was markedly lower than at previous rallies, which had attracted hundreds of thousands of people.
As it has before, today's rally opened with speeches by the country's spiritual leaders, including Christian priests, a rabbi and a mufti who called for a national unity and stressed the protesters' right to have the government they want.
Oleh Tyahnybok, head of the opposition national party Svoboda notorious for his racist rhetoric, emphasized that Ukrainians in the west and the east should unite to fight for their rights.
Most demonstrators in Kiev come from western and central regions, while many people in the mostly Russian-speaking east and the south back closer ties with Moscow.
Also Read
The demonstrations were sparked by President Viktor Yanukovych's decision last month to spike the EU deal in favour of closer ties with Russia. The move angered many Ukrainians, who hoped that closer ties with the EU would help end centuries of Russia's domination.
The protests were galvanised by a brutal police crackdown on November 30, but Yanukovych's government has since limited the use of force in an apparent hope that protests will fizzle out.
Kiev's Independence square, or the Maidan, was filled with wood fire smoke today coming from field kitchens and stoves that protesters installed in the tents they have been living at for weeks. The city's main street, Khreshchatyk, has been barricaded with wooden planks, sacks filled with snow, and car tires since November's crackdown.