The airline would be starting its flight connecting Tiruchirapalli with Delhi and Mumbai via Chennai from January 15.
"With the launch of these flights, Tiruchirapalli now would be connected to national network," SpiceJet Chief Executive Officer Neil Mills said.
The airline would deploy its newly-acquired Bombardier Q400 aircraft for enhancing connectivity to Tier II and Tier III cities.
The Bombardier Q400 NextGen turboprop aircraft can accommodate 78 passengers.
SpiceJet flies to 40 domestic and six international destinations.