The Port Authority of New York and New Jersey says the spire's installation was completed this morning.
The 541 metres is symbolic of the year 1776, when the US declared its independence.
Pieces of the spire were transported to the roof of the building last week. It weighs 758 tons.
The 124.36-metre spire will serve as a world-class broadcast antenna. An LED-powered light emanating from it will be seen from miles away.