Mauricio Pochettino's side struggled at England's national stadium when playing European home fixtures there last season, and have so far fared no better in the Premier League.
Chelsea's 2-1 victory last Sunday means that Spurs have lost eight of their last 11 matches at Wembley, their home for the season as White Hart Lane is rebuilt and expanded.
Even though the performance was good in that match, that defeat was a setback for Tottenham, who had looked comfortable in winning 2-0 at Newcastle on the opening weekend of the season.
Manager Mauricio Pochettino said: "I think we know each other very well and they were good against Chelsea but struggled against West Brom.
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"One thing is sure they will be motivated to play at Wembley against us. For us it is important to play well. It will be tough."
Tottenham made their first signings of the summer by paying Ajax a club-record ?42million to sign Davinson Sanchez, the Colombian defender, and back-up goalkeeper Paula Gazzaniga has arrived from Southampton.
"The important thing is that he is so young, at 21 years old," said Pochettino.
"He is one of the most important and talented young players in Europe. He is so fast. We are so pleased to sign him and have him with us."
Pochettino has indicated that more signings are needed before the transfer window closes next Thursday, in order to to ensure the club are ready for the twin demands of Premier League and Champions League football this season.
He added: "We need to sign a few more players, and a few players need to leave the club.
Tottenham remain without Georges-Kevin Nkoudou (foot), Danny Rose (knee) and Erik Lamela (hip), even though all three are progressing in their recovery from injury.
Burnley have only played at Wembley five times in their entire history, the most recent of which was the 2009 Championship play-off final, when they beat Sheffield United to end a 33-year exile from the top division.
Two relegations and two promotions have followed since, but last season's survival has given manager Sean Dyche the chance to build a solid base in the Premier League.
That match was a fraught affair, with midfielder Ashley Westwood forced to tackle a fan who ran on to the pitch and shoved team-mate James Tarkowski, while a nine-year-old fan was injured by a flare. Police have charged four supporters over the trouble.
Dyche, for his part, is glad to be able to concentrate on the football once more.
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