Stalin, also Opposition leader in the Tamil Nadu Assembly, was detained along with scores of supporters when he staged a rail blockade at suburban Mambalam Railway Station.
Other DMK leaders, including party MP Kanimozhi and former union minister Dayanidhi Maran, as also senior leaders, participated in the state-wide rail roko held by the party.
A large number of autorickshaws also kept off the roads across Tamil Nadu, even as fewer buses plied.
The cinema industry also embarked on a silent protest, with both actors and actresses wearing black outfits.
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Meanwhile protesters, especially at Alangalllur in Madurai, vowed to continue their protest till a permanent solution was found to the vexed issue and hold the bull taming sport in Tamil Nadu, despite the Chief Minister's announcement.
In Rameswaram, all shops remained closed and pilgrims had a tough time getting even food in the island.
Following the protests, Southern Railway announced full and partial cancellation of some trains, besides diversion of others.
Union Railway Minister Suresh Prabhu asked the protestors not to cause inconvenience to the poor.
"Rail is preferred mode of transport for poor, common people. Please don't disrupt traffic, cause inconvenience to those who can't afford other," he said in a tweet.