Researchers found over 7,000 stone tools showing a distinct upgrade in stone-shaping techniques, including advanced blades, points and scrapers.
The findings, published in the journal Nature, suggest that modern stone tools were being made in India 250,000 years earlier than previously thought.
The tools may have been made by an archaic species of hominin, rather than modern humans, researchers said.
According to Chris Clarkson from the University of Queensland in Australia, the earliest evidence for modern humans was found in Morocco and dated between 281,000 and 349,000 years old.
More From This Section
In 1999, archaeologists Kumar Akhilesh and Shanti Pappu from the Sharma Centre for Heritage Education in Tamil Nadu began excavating the Attirampakkam archaeological site.
They uncovered more than 7,000 stone tools along the way, 'ABC News' reported.
The haul contained tools produced by a more advanced method typical of Middle Stone Age culture, known as the Levallois technique, Pappu said.
The researchers said that the newly discovered stone tools are the oldest Middle Stone Age artifacts known from India by far.
Previously discovered Indian Middle Stone Age artifacts have been dated to about 140,000 to 46,000 years ago.
Outside Africa, the oldest modern human fossil was a partial jaw bone found in Israel. It was dated to about 185,000 years old, according to researchers.