While customers could not get their cheques cleared, services like cash withdrawal, cash and cheque deposits were also hit.
Several harried customers faced inconvenience as they found many branches of public sector banks (PSBs) closed by the protesting employees.
The industrial sector including MSME and large enterprises of Punjab and Haryana were the worst hit due to the strike.
However, major private sector banks continued to function.
The protesting employees held rallies and demonstrations at several places including Ludhiana to press for their demands.
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"But we are left with no option except to go on strike," he said.
He said bank officials' major demands include stopping mergers, consolidation and privatisation of banks, criminal action against wilful defaulters of bank loans, implement recommendations of Parliamentary Committee on recovery of NPAs, abolish bank board bureau, among others.
In Phagwara, banks officials raised slogans against Central government and its "anti-people" banking policies.
Punjab Bank Employees Federation Phagwara Chairman V K Verma and Secretary Narinder Singh raised slogans against government's "double standard" on non-performing asset(NPA) policy.