A total of 4,882 students from the India International School of the PACE Education Group participated in the event by wearing the colours of the UAE National flag, the Gulf News reported.
The school management said the attempt was held to mark Childrens Day in India, the birth anniversary of first Indian prime minister Jawaharlal Nehru, who was very fond of children.
"It is a great achievement for our students. It is probably a once-in-a-lifetime experience for them," school principal Manju Reji said.
Because the UAE National Day is approaching, she said the school decided to use the flag colours to have a joint celebration of both Childrens Day and UAE National Day.
All the students from grade one to grade eight who participated in the attempt will receive a certificate from the Guinness World Records.
The adjudicator for Guinness World Records Ahmed Gabr declared India International School "Officially Amazing" by setting the World Record for the "Largest Human Image of a Boat with 4882 students", the school said.