The confirmation comes after Myanmar on Wednesday set November 8 as the day for polls expected to be the most important election in a generation.
"We have decided to take part in the election," Suu Kyi told reporters at a press conference at her residence in the capital Naypyidaw, vowing the party would "amend the constitution" that bars her from the presidency if it wins the polls.
But despite the loss the Nobel laureate had vowed not to "back down" from the election and her opposition is tipped to make huge gains at the ballot box if the vote is free and fair.
The NLD won nationwide polls in 1990 by a landslide, while Suu Kyi was under house arrest. But it was prevented from taking power by the military, who had plunged the country into decades of isolation.