After rejecting Talwars' petition, Additional Sessions Judge Shyam Lal fixed September 26 for the start of final arguments in the case.
In their petition, the dentist couple who are being tried for murders of their daughter Aarushi and domestic help Hemraj had appealed the court that they wanted cross-examination of Dr Mahapatra "as several questions are yet to be asked".
"Our application has been rejected by the court," said Manoj Sisodia, counsel of Rajesh and Nupur Talwar.
So far, all the defence and prosecution witnesses have been examined in the Ghaziabad's CBI court and final arguments will start from Thursday.