Paul Reubens is reviving the Pee-wee Herman character for a Netflix original film produced by Judd Apatow and written by Arrested Development's Paul Rust.
The movie follows Pee-wee as he takes his first ever holiday after a chance encounter with a mysterious stranger.
Buck, 39, has been lined up for a prominent role in "Pee-wee's Big Holiday", reported Deadline.
The actress is best known for playing the eccentric Ginger in "True Blood", and has also made memorable appearances in "The X-Files", "Bones" and "Criminal Minds".
"Pee-wee's Big Holiday" will mark the feature film directorial debut of John Lee (Broad City).