The team had a five-and-half-hour practice, supervised by chief coach Duncan Fletcher, bowling coach Joe Dawes and fielding coach Evan Speechly, at the Brabourne Stadium.
The rigorous session started with batsmen coming in pairs at the centre of the pitch. Later, most of them spent a few minutes at the nets.
All batsmen, who are expected to make up the first Test's line-up at Ahmedabad, had a decent stint against the likes of Umesh Yadav, Zaheer Khan, Ishant Sharma, Pragyan Ojha, Harbhajan Singh, R Ashwin and local net bowlers.
Skipper Mahendra Singh Dhoni also rolled his arms over and bowled a few deliveries. Off-spinner Harbhajan Singh, who missed yesterday's session, was back in action as he spent time fielding, bowling and batting.
There was a slight injury scare for senior batsman Sachin Tendulkar, who was fielding alongside Cheteshwar Pujara and R Ashwin at the start of the day, when he fell off balance and skidded on the ground as the ball hit his hand.
He excused himself from fielding but was back half an hour later and batted for nearly an hour without showing any signs of discomfort. The maestro followed it up with 25 minutes at the nets, the longest by any batsman.
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Tendulkar's son Arjun had come to see the team's practice and watched Yuvraj Singh and Virat Kohli's batting.
After the conclusion of the three-day preparatory camp, the team will depart on November 12 for the series opener at the Motera Stadium.
The same 15 has been retained for the second match of the rubber at the Wankhede Stadium here from November 23. The last two matches of the series will be played at Kolkata (December 5-9) and Nagpur (December 13-17).
The England team is already in Ahmedabad playing their third and final warm-up tour game against Haryana.