A group of migrants on Saturday tried to bring down a fence in a desperate attempt to bust through the border into Greece while others hurled rocks at Greek police.
Greek authorities responded, firing volleys of tear gas at the youths.
At least two migrants were injured in the latest clash between Greek police and migrants gathered on the Turkish side of a border crossing near the Greek village of Kastanies.
As in previous confrontations this week, officers in Greece fired tear gas to impede the crowd and Turkish police fired tear gas back at their Greek counterparts.
Groups of mostly young men tied ropes onto the fence in an attempt to tear it down. Some shouted "Allah is Great" while others shouted open the border."
The announcement came hours after EU foreign ministers meeting in Croatia on Friday criticized Turkey, saying it was using the migrants' desperation "for political purposes."
While crediting Turkey for hosting millions of migrants and refugees, European foreign ministers said the bloc strongly rejects Turkey's use of migratory pressure for political purposes."
Their masks have fallen," he said. The ruthlessness of those who gave lectures on humanity has become evident."
Soylu has said Erdogan instructed Turkish authorities to prevent migrants from attempting to reach the Greek islands in dinghies to avoid human tragedies."