Suu Kyi also called for calm and stability as the campaign period nears its end ahead of the November 8 election.
Tens of thousands of ecstatic National League for Democracy supporters swarmed onto a large playing field, waiting for hours in the blazing sunshine for Suu Kyi to make her entrance.
She had hoped to hold the rally in the center of Yangon, near the revered Shwedagon Pagoda, reviving memories of her first-ever political speech in 1988, but city authorities refused her request.
Just days after an NLD member was wounded in a stabbing at another rally, Suu Kyi asked the crowds to maintain stability right up to the end of campaigning. Without naming names, she said that "there are some who are thinking to use bad ways to try to win."
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Suu Kyi spent 15 years under house arrest under the former military dictatorship. She was finally released five years ago.
The junta stepped back from power in 2011 with the election of President Thein Sein, and the country has moved toward democratization, though the military still maintains a powerful position.