Raju Dewan, died this morning in a private nursing home, whereas, his wife Loni Dewan had also died of the vector-borne disease on Tuesday.
Another person, identified as Kandarpa Deka, also suffering from dengue died on his way to the hospital yesterday.
Kamrup(Metro) Joint Director of health services Sailen Choudhury said so far 18 cases of dengue have been reported in the district.
The health department has set up blood sample collection centres in different parts of the district and have appealed to people to go for the tests if they were suffering from high fever, headaches, joint and muscle pains.
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The others suffering from the disease are admitted to different nursing homes.
Guwahati Municipal Corporation Mayor Abir Patra said that fogging has been initiated in different parts of the town to ensure that the disease does not spread and assume an alarming situation.
Dengue cases were mostly reported from Zoo Road Tinaili, Narengi, Noonmati, Odalbakra and Maligaoan areas of Guwahati, sources added.