According to sub-inspector G Srinivas of the Petbasheerabad police, the incident happened at around 1:30 am.
One Anil Kumar, who was driving the motorcycle, lost control and crashed into the divider, he said.
"Anil Kumar and one Ratan died on the spot while the third person on the motorbike, Amanraj, was declared dead on arrival by doctors after he was rushed to a hospital," said Srinivas.
All three, he said, were between aged 21 to 22 years.