The accident took place at around 2.05 PM. The speeding car, coming from the direction of Alipore Zoo, jumped a red signal near the AJC Bose and Belvedere Road crossing and hit two bikers, police said.
The car then slammed into the roadside guardrails and climbed the footpath hitting more than 13 pedestrians, they said.
One biker and two others were killed on the spot.
Police have arrested the driver of the car, who was badly injured and is being treated along with others at the nearby state-run SSKM hospital.