The family members hailing from Valsad were travelling in an SUV which collided with a truck near Chousale village on Vani-Saputara Road near here yesterday, they said.
The truck was proceeding towards Saputara in Gujarat, while the car was on its way to Nashik.
The deceased were identified as Babubhai Chhannabhai Patel (61), his wife Pushaben (55) and Dhirubhai (40), their relative, the police said.
The injured were admitted to a Government hospital in Nashik.