The stock after listing at Rs 662, a sharp gain of 48.43 per cent over the issue price, ended the day at Rs 618.10, up 38.59 per cent, on BSE.
Intra-day, it zoomed 49.19 per cent to Rs 665.40.
On NSE, it soared 38.74 per cent to close at Rs 618.80.
This is the biggest gain by any company on its market debut so far this year.
The company's market valuation currently stands at Rs 3,320.65 crore.
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The issue was open for bidding on April 27-29.
The firm, which runs a diagnostics laboratory chain, has come out with the public issue of up to 10,744,708 shares.
JM Financial Institutional Securities, Edelweiss Financial Services and ICICI Securities managed the issue.
Thyrocare competes with diagnostics chains such as Dr Lal PathLabs, SRL Diagnostics, Metropolis Healthcare and Apollo Clinic.
Many healthcare firms like Alkem Laboratories, Dr Lal PathLabs and Narayana Hrudayalaya came out with their IPOs last year while HealthCare Global Enterprises got listed in March.