Field Director R S Murthy said the tigress tagged "T-6" and her three of her cubs were spotted for the first time at Hinotia range of the reserve recently.
The cubs are about two-months-old.
Murthy said since three cubs have been spotted, the tiger population in Panna Tiger Reserve has risen to 26.
Tigress 'T-6' had delivered a cub in August last year but couldn't rear it.
The Panna Tiger Reserve had made news in 2009 after it became devoid of any big cats owing to various reasons, that included poaching.
Tigress 'T-6' was relocated to the reserve from the Pench Tiger Reserve in January 2014 under this scheme.