A win for the South Americans would send Cesare Prandelli's side crashing out at the first hurdle, four years after bowing out at the group stage as then world champions in South Africa.
The Azzurri require just a draw to go through, but after a lifeless display in a 1-0 defeat to Costa Rica, captain Gianluigi Buffon hinted that the hot and humid conditions have taken their toll.
"I think a lot of European teams are struggling compared to the Latin Americans.
"But that isn't an excuse. The climate is the same for all of our opponents."
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If there is belief that Latin Americans sides are coping better than teams from Europe in such a testing climate, Group D is arguably a good indicator.
Costa Rica host England on Tuesday looking for their third consecutive group win having fought back from a goal down to stun Uruguay 3-1, before outplaying a visibly jaded Italy in Recife on Friday.
Uruguay bounced back from that shock reverse with a far improved performance to beat England 2-1 thanks to a Luis Suarez double.
"They've managed to relaunch their campaign by beating England and now the whole of Uruguay believes they can beat us and go through," added Buffon, who said Italy will have to be wary of Liverpool striker Suarez.
"We know plenty about (striker Edinson) Cavani thanks to his time at Napoli. But we know less about Suarez."
"We need to show some strength and pride and come up with a win. We need cool heads but also to be highly motivated.
"If we end up going out in the first round, it will be a failure.