In an interview with Screen Rant, the 55-year-old actor discussed the rumours, which started when a Twitter user shared a screen shot in August of Cruise's backside in a scene from the film.
In the tweet, the user wrote, "Hello, please, I present the theory that for one single shot in Valkyrie (2008), at 5:12, Tom Cruise wears a fake butt. Observe."
"There was no prosthetic in Valkyrie," Cruise said.
Cruise added that the butt featured in several scenes throughout his new film "American Made" is also all him.