The party, which has 19 members in the Lok Sabha, gave a letter withdrawing support to the government to President Pranab Mukherjee.
Minutes earlier, six Trinamool Congress Ministers submitted their resignations to Prime Minister Manmohan Singh who expressed sadness over their decision.
The Ministers -- Mukul Roy, Sudip Bandhyopadhay, Sultan Ahmed, Saugata Roy, Sisir Adhikary and C M Jatua -- went to the Prime Minister's 7, Race Course Road residence to submit their resignations.
Straight from there, they went to Rashtrapati Bhavan for give letter withdrawing support.
"We, the six Trinamool Congress Ministers of the Council of Ministers, including Cabinet Minister, went to the Prime Minister's residence at 4 PM and we handed over to him individual letters of resignations of all the six Ministers," said Saugata Roy.
"Now, we have come to Rashtrapati Bhavan and handed over a letter to President Pranab Mukherjee, signed by Mukul Roy, Chairman of Trinamool Congress Parliamentary Party, withdrawing our support to UPA-II government... The President has accepted our letter of withdrawal of support," he said.
Earlier, while receiving the resignation letters, the Prime Minister said he was "sad" that they were quitting, Bandhyopadhay said. (MORE)