Desai and other women activists arrived at the temple at around noon and offered prayers from outside the 'garbha griha' amid tight police arrangements at the place, Inspector P H Sapkale, in-charge of Panchavati police station, said.
Later, she and the others were escorted in a police van some distance away from the temple, from where they left for Pune.
Last Thursday, Desai had to return without offering prayers following strong opposition from locals, priests and trustees of the temple.
Some city residents had yesterday held a meeting at a hall behind the temple and strongly opposed Desai's earlier decision to enter the sanctum sanctorum of the temple today.
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Notably, men are also not allowed to enter the sanctum sanctorum where only priests are permitted to perform the regular puja.
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Meanwhile, people gathered and began shouting slogans when Desai entered the temple and after she came out.
Police used lathis to disperse a few devotees from the area.
Later, Desaiand other activists returned to Pune.