Trump's diatribe came after the 64-year-old Winfrey in a TV show interviewed several Michigan voters about the president's first year in office.
The talk show queen asked the voters about the presidents tax plan, his reported reference to "shithole countries" and about Americas eroding reputation around the globe.
Trump claimed that Winfrey posed "biased and slanted" questions to the panel on the news programme.
"The questions were biased and slanted, the facts incorrect. Hope Oprah runs so she can be exposed and defeated just like all of the others!" he said.
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Winfrey accepted the Golden Globes' Cecil B DeMille Award for lifetime achievement last month, and gave an acceptance speech that explored issues of race and gender among other hot social and political topics.
Her speech gave rise to speculation of a potential presidential bid in 2020, but Winfrey has denied any such plans.
"I am actually humbled by the fact that people think that I could be a leader of the free world, but it's just not in my spirit - its not in my DNA," Winfrey told news magazine show 'Sixty Minutes' last week.
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