The move came hours after the release of an audio file on YouTube, purporting to be of a security meeting in which top government, military and spy officials discuss a possible scenario for military action inside Syria.
Prime Minister Recep Tayyip Erdogan's government informed mobile phone operators and internet service providers of its decision to block YouTube, the Hurriyet newspaper reported on its website.
The move came a day after a court ordered a suspension of the Twitter ban.
It added that it was a "natural practice" by the state to discuss how to protect Turkish property from "terrorist elements" but added that some part of the conversation had been "distorted".
Erdogan, whose party faces key local elections Sunday, has been dogged by a string of leaks, including apparent wire taps suggesting a major corruption scandal, which have gone viral on Twitter and other social media.