In the western city of Izmir, about 100 kilometres (60 miles) from Soma, which was hit hard by Tuesday's mine explosion, police fired tear gas and water cannon at around 20,000 protesters on yesterday.
Turkey's four biggest unions held a one-day strike, saying workers' lives were being jeopardised to cut costs, and demanding those responsible for the collapse of the coal mine be brought to account.
"Hundreds of our workers have been left to die from the very beginning by being forced to work in cruel production processes to achieve maximum profits," they said in a joint statement, calling on people to wear black.
In Izmir, the 61-year-old head of one of the main unions Kani Beko was hospitalised after violent clashes with riot police.
Also Read
In Ankara, police fired tear gas and water cannon on around 200 protesters accusing the government and mining industry of negligence.
Erdogan has rejected claims of government culpability, saying that "such accidents happen".
He compared the collapse to 19th-century mining disasters, saying that "204 people died in the UK in 1862 and 361 people in 1864", in an apparent attempt to downplay its severity.
Photographs of his advisor Yusuf Erkel kicking a protester in Soma sparked outrage on social media.
Defending his actions, Erkel told Hurriyet newspaper: "He attacked and insulted me as well as the prime minister. Should I have stayed silent?"
At a protest in central Istanbul yesterday near the city's emblematic Taksim square, the site of weeks of violent anti-government protests last year, angry demonstrators held the country's leaders responsible for the disaster.